【Labs spotlight】 Kudo Laboratory（until Mar. 2017）

The Department has a variety of laboratories for Life Science and Technology, in which cutting-edge innovative research is being undertaken not only in basic science and engineering but also in the areas of medicine, pharmacy, agriculture, and multidisciplinary sciences.

This "Spotlight" series features a laboratory from the Department and introduces you to the laboratory's research projects and outcomes. This time we focus on Kudo Laboratory.

※Professor Kudo was retired on March 31, 2017.

Research interest

We have been studying the molecular system of bone formation and remodeling employing medaka as an animal model, and performed the next 4 projects.

1. 1. To find the mechanism of pattern formation for vertebral bone during development
2. 2. To find the collaboration of osteoblasts and osteoclasts in bone remodeling
3. 3. To reveal the mechanism of bone loss under microgravity
4. 4. To find the function of periostin in fin development and regeneration

Research findings

Selected publications

[1] Chatani, M., Morimoto, H., Takeyama, K., Mantoku, A., Tanigawa, N., Kubota, K., Suzuki, H., Uchida, S., Tanigaki, F., Shirakawa, M., Gusev, O., Sychev, V., Takano, Y., Itoh, T. and Kudo, A. Acute transcriptional regulation in osteoblasts/osteoclasts immediately after exposure to microgravity, uncovered by cell imaging in medaka.Sci. Rep. 6: 39545 (2016)

[2] Takeyama, K., Chatani, M., Inohaya, K. and Kudo, A. TGFβ-2 signaling is essential for osteoblast migration and differentiation during fracture healing in medaka fish. Bone 86: 68-78 (2016)

[3] Mantoku, A., Chatani, M., Aono, K., Inohaya, K. and Kudo, A. Osteoblast and osteoclast behaviors in the turnover of attachment bones during medaka tooth replacement. Dev. Biol. 409: 370-381 (2016)

[4] Chatani, M., Mantoku, A., Takeyama, K., Abduweli,D., Sugamori, Y., Aoki, K., Ohya, K., Suzuki, H., Uchida, S., Sakimura, T., Kono, Y., Tanigaki, F., Shirakawa, M., Takano, Y. and Kudo, A. Microgravity promotes osteoclast activity in medaka fish reared at the international space station. Sci. Rep. 5: 14172 (2015)

[5] Taimatsu, K., Takubo, K., Maruyama, K., Suda, T. and Kudo, A. Proliferation following tetraploidization regulates the size and number of erythrocytes in the blood flow during medaka development, as revealed by the abnormal karyotype of erythrocytes in the medaka TFDP1 mutant. Dev. Dyn. 244: 651-668 (2015)

[6] Takeyama, K., Chatani, M., Takano, Y. and Kudo, A. In-vivo imaging of the fracture healing in medaka revealed two types of osteoclasts before and after the callus formation by osteoblasts. Dev. Biol. 394: 292-304 (2014)

[7] Ito, K., Morioka, M., Kimura, S., Tasaki, M., Inohaya, K. and Kudo, A. Differential reparative phenotypes between zebrafish and medaka after cardiac injury. Dev. Dyn. 243: 1106-1115 (2014)

[8] Iida, Y., Hibiya, K., Inohaya, K. and Kudo, A. Eda/Edar signaling guides fin ray formation with preceding osteoblast differentiation, as revealed by analyses of the medaka all-fin less mutant afl. Dev. Dyn. Doi: 10.1002/DVDY.24120 (2014)

[9] Fujita, M., Mitsuhashi, H., Isogai, S., Nakata, T., Kawakami, A., Nonaka, I., Noguchi, S., Hayashi, Y. K., Nishino, I. and Kudo, A. Filamin C plays an essential role in the maintenance of the structural integrity of cardiac and skeletal muscles, revealed by the medaka mutant zacro. Dev. Biol. 361: 79-89 (2012)

[10] Chatani, M., Takano, Y. and Kudo, A. Osteoclasts in bone modeling, as revealed by in vivo imaging, are essential for organogenesis in fish. Dev. Biol. 360: 96-109 (2011)

[11] Moriyama, A., Inohaya, K., Maruyama, K. and Kudo, A. Bef mutant reveals the essential role of c-myb in both primitive and definitive hematopoiesis. Dev. Biol. 345: 133-143 (2010)

[12] Inohaya, K., Takano, Y. and Kudo, A. Production of wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka. Development 137: 1807-1813 (2010)

[13] Ohisa, S., Inohaya, K., Takano, Y. and Kudo, A. sec24d encoding a component of COPII is essential for vertebra formation, revealed by the analysis of the medaka mutant, vbi. Dev. Biol. 342: 85-95 (2010)

[14] Hibiya, K., Katsumoto, T., Kondo, T., Kitabayashi, I. and Kudo, A. Brpf1, a subunit of the MOZ histone acetyl transferase complex, maintains expression of anterior and posterior Hox genes for proper patterning of craniofacial and caudal skeletons. Dev. Biol. 329: 176-190 (2009)

• Research Laboratories and Subjects
• Gravitational biology: Real time imaging and transcriptome analysis of medaka aboard space station | Tokyo Tech News
• Fish reveal details of bone density loss during space missions | Tokyo Tech News
• "Fishonauts" to enter space again | Tokyo Tech News