【Labs spotlight】 Kobatake and Mie Laboratory

The Department has a variety of laboratories for Life Science and Technology, in which cutting-edge innovative research is being undertaken not only in basic science and engineering but also in the areas of medicine, pharmacy, agriculture, and multidisciplinary sciences.

This "Spotlight" series features a laboratory from the Department and introduces you to the laboratory's research projects and outcomes. This time we focus on Kobatake and Mie Laboratory.

Degree	Professor Eiry Kobatake : PhD 1990, Tokyo Institute of Technology Associate Professor Masayasu Mie : PhD 2000, Tokyo Institute of Technology
Areas of Research	Professor Eiry Kobatake : Construction of super biofunctional protein materials Associate Professor Masayasu Mie : Construction of biomolecular tools
Keywords	Professor Eiry Kobatake : protein engineering, cellular and tissue engineering, biomaterials, biosensing Associate Professor Masayasu Mie : biomaterials, protein engineering, cellular engineering, biosensing
Website	Kobatake and Mie Laboratory

Research interest

Research result

Our publication list is available from our web site.

Selected publications

• [1] Ikeda Y, Mashimo Y, Mie M, Kobatake E. Design of luciferase-displaying protein nanoparticles for use as highly sensitive immunoassay detection probes. Analyst. 2016;141:6557-63.
• [2] Mie M, Naoki T, Kobatake E. Development of a Split SNAP-CLIP Double Labeling System for Tracking Proteins Following Dissociation from Protein Protein Complexes in Living Cells. Anal Chem. 2016;88:8166-71.
• [3] Adnan N, Mie M, Hague A, Hossain S, Mashimo Y, Akaike T, Kobatake E. Construction of a Defined Biomimetic Matrix for Long-Term Maintenance of Mouse Induced Pluripotent Stem Cells. Bioconjugate Chem. 2016;27:1599-605.
• [4] Siew S, Kaneko M, Mie M, Kobatake E. Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation. J Mater Chem B. 2016;4:2512-8.
• [5] Assal Y, Mizuguchi Y, Mie M, Kobatake E. Growth Factor Tethering to Protein Nanoparticles via Coiled-Coil Formation for Targeted Drug Delivery. Bioconjugate Chem. 2015;26:1672-7.
• [6] Mie M, Naoki T, Kobatake E. Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system. Anal Biochem. 2015;477:53-5.
• [7] Matsumoto R, Hara R, Andou T, Mie M, Kobatake E. Targeting of EGF-displayed protein nanoparticles with anticancer drugs. J Biomed Mater Res B. 2014;102:1792-8.
• [8] Mie M, Sasaki S, Kobatake E. Construction of a bFGF-tethered multi-functional extracellular matrix protein through coiled-coil structures for neurite outgrowth induction. Biomed Mater. 2014;9.
• [9] Assal Y, Mie M, Kobatake E. The promotion of angiogenesis by growth factors integrated with ECM proteins through coiled-coil structures. Biomaterials. 2013;34:3315-23.
• [10] Mie M, Kaneko M, Henmi F, Kobatake E. Induction of motor neuron differentiation by transduction of Olig2 protein. Biochem Bioph Res Co. 2012;427:531-6.
• [11] Mashimo Y, Maeda H, Mie M, Kobatake E. Construction of Semisynthetic DNA-Protein Conjugates with Phi X174 Gene-A* Protein. Bioconjugate Chem. 2012;23:1349-55.
• [12] Akter F, Mie M, Grimm S, Nygren PA, Kobatake E. Detection of Antigens Using a Protein-DNA Chimera Developed by Enzymatic Covalent Bonding with phiX Gene A. Anal Chem. 2012;84:5040-6.
• [13] Mie M, Naoki T, Uchida K, Kobatake E. Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells. Analyst. 2012;137:4760-5.
• [14] Kobatake E, Takahashi R, Mie M. Construction of a bFGF-Tethered Extracellular Matrix Using a Coiled-Coil Helical Interaction. Bioconjugate Chem. 2011;22:2038-42.
• [15] Mashimo Y, Mie M, Suzuki S, Kobatake E. Detection of small RNA molecules by a combination of branched rolling circle amplification and bioluminescent pyrophosphate assay. Anal Bioanal Chem. 2011;401:221-7.
• [16] Nakamura M, Mie M, Kobatake E. Construction of a functional IgG-binding luciferase fusion protein for the rapid detection of specific bacterial strains. Analyst. 2011;136:71-2.
• [17] Mie M, Sugita R, Endoh T, Kobatake E. Evaluation of small ligand-protein interactions by using T7 RNA polymerase with DNA-modified ligand. Anal Biochem. 2010;405:109-13.
• [18] Nakamura M, Yamaguchi K, Mie M, Nakamura M, Akita K, Kobatake E. Promotion of Angiogenesis by an Artificial Extracellular Matrix Protein Containing the Laminin-1-Derived IKVAV Sequence. Bioconjugate Chem. 2009;20:1759-64.
• [19] Fujita Y, Mie M, Kobatake E. Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain. Biomaterials. 2009;30:3450-7.
• [20] Nakamura M, Mie M, Mihara H, Nakamura M, Kobatake E. Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells. Biomaterials. 2008;29:2977-86.

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