【Labs spotlight】 Fujita Laboratory

Office	Room 210, S2 building, Suzukakedai campus
Degree	PhD(Doctor of Engineering) 2009, Waseda University
Areas of Research	Cell biology
Keywords	Organelle, Muscle, Drosophila, Autophagy, Lysosome
Web site	Fujita Lab(Japanese)

Office

Room 210, S2 building, Suzukakedai campus

Degree

PhD(Doctor of Engineering) 2009, Waseda University

Areas of Research

Cell biology

Keywords

Organelle, Muscle, Drosophila, Autophagy, Lysosome

Web site

Fujita Lab(Japanese)

Research interest

Differentiated muscle cells have highly organized membranous organelles, such as Transverse (T)-tubules required for excitation-contraction coupling. Their roles are established; however, the mechanisms shaping the organelles are largely unknown. Our study aims to elucidate the mechanisms to form and remodel the membranous organelles in muscle cells using Drosophila and mammalian cultured cells. Further, we are trying to identify causative genes for hereditary human myopathies using fly genetics.

We are also working on analyzing the mechanism and function of autophagy-mediated phenomena through a unique approach using Drosophila.

図2 ショウジョウバエの筋細胞

図3 ハエの変態に伴う筋細胞のリモデリング

Selected publications

1.Homma, Y., Kinoshita, R., Kuchitsu, Y., Wawro, P. S., Marubashi, S., Oguchi, M., Ishida, M., Fujita, N., Fukuda, M. Comprehensive knockout analysis of the Rab family GTPases in epithelial cells.J Cell Biol (2019) 218, 2035-2050.
2.Kuchitsu, Y., Homma, Y., Fujita, N.^#, and Fukuda, M.^# Rab7 knockout unveiled regulated autolysosome maturation induced by glutamine starvation J Cell Sci (2018) 131, jcs215442 (# co-corresponding authors)
3.Fujita, N.^#, Huang, W., Lin, T.H., Groulx, J.F., Jean, S., Nguyen, J., Kuchitsu, Y., Koyama-Honda, I., Mizushima, N., Fukuda, M., and Kiger, A.A.^# Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. Elife (2017) 6. e23367 (# co-corresponding authors)
4.Fujita, N.^*, Morita, E.^*, Itoh, T., Tanaka, A., Nakaoka, M., Osada, Y., Umemoto, T., Saitoh, T., Nakatogawa, H., Kobayashi, S., Haraguchi, T., Guan, J.L., Iwai, K., Tokunaga, F., Saito, K., Ishibashi, K., Akira, S., Fukuda, M., Noda, T., and Yoshimori, T. Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin. J Cell Biol (2013) 203, 115-128. (* co-first authors)
5.Hamasaki, M., Furuta, N., Matsuda, A., Nezu, A., Yamamoto, A., Fujita, N., Oomori, H., Noda, T., Haraguchi, T., Hiraoka, Y., Amano, A., and Yoshimori, T. Autophagosomes form at ER-mitochondria contact sites. Nature (2013) 495, 389-393.
6.Fujita, N., Saitoh, T., Kageyama, S., Akira, S., Noda, T., and Yoshimori, T. Differential involvement of Atg16L1 in Crohn disease and canonical autophagy: analysis of the organization of the Atg16L1 complex in fibroblasts. J Biol Chem (2009) 284, 32602-32609.
7.Hayashi-Nishino, M., Fujita, N., Noda, T., Yamaguchi, A., Yoshimori, T., and Yamamoto, A. A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation. Nat Cell Biol (2009) 11, 1433-1437.
8.Saitoh, T.^*, Fujita, N.^*, Hayashi, T., Takahara, K., Satoh, T., Lee, H., Matsunaga, K., Kageyama, S., Omori, H., Noda, T., Yamamoto, N., Kawai, T., Ishii, K., Takeuchi, O., Yoshimori, T., and Akira, S. Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response.
Proc Natl Acad Sci U S A (2009) 106, 20842-20846. (^* co-first authors)
9.Saitoh, T. ^*, Fujita, N.^*, Jang, M.H., Uematsu, S., Yang, B.G., Satoh, T., Omori, H., Noda, T., Yamamoto, N., Komatsu, M., Tanaka, K., Kawai, T., Tsujimura, T., Takeuchi, O., Yoshimori, T., and Akira, S. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature (2008) 456, 264-268. (^* co-first authors)
10.Fujita, N., Hayashi-Nishino, M., Fukumoto, H., Omori, H., Yamamoto, A., Noda, T., and Yoshimori, T. An Atg4B mutant hampers the lipidation of LC3 paralogues and causes defects in autophagosome closure. Mol Biol Cell (2008) 19, 4651-4659.
11.Fujita, N., Itoh, T., Omori, H., Fukuda, M., Noda, T., and Yoshimori, T. The Atg16L complex specifies the site of LC3 lipidation for membrane biogenesis in autophagy. Mol Biol Cell (2008) 19, 2092-2100.