Life Science and Technology News
Green chemistry by enzymes for organic synthesis
The Department has a variety of laboratories for Life Science and Technology, in which cutting-edge innovative research is being undertaken not only in basic science and engineering but also in the areas of medicine, pharmacy, agriculture, and multidisciplinary sciences.
This "Spotlight" series features a laboratory from the Department and introduces you to the laboratory's research projects and outcomes. This time we focus on Matsuda Laboratory.
Life Science and Technology
Associate Professor Tomoko Matsuda
|Degree||2000, Kyoto University Doctor of science|
|Areas of Research||Biocatalysis, Green Chemistry, Asymmetric Organic Synthesis.|
|Keywords||Enzyme, Alcohol dehydrogenase, Lipase, Carbon Dioxide, Supercritical Fluid, Green Chemistry, Chiral Synthesis.|
Our research interest is to promote green chemistry by using biocatalytic reactions; for example, asymmetric reduction of ketones by alcohol dehydrogenase, kinetic resolutions of racemic alcohol by lipase using CO2-based solvents, and carboxylation using decarboxylase using CO2 as a solvent. Because of homo-chirality in biology on the earth, for instance amino acids which are composed of only left-handed or L-isomer, the discovery and development of stereoselective organic synthetic methods for pharmaceutical and agrochemicals are very important. Chemical and biological catalysts have been developed throughout several decades. To synthesize them in environmentally friendly methods, enzymes are being used as catalysts.
We has utilized pressurized CO2 (liquid or supercritical CO2), which has numerous positive impacts on green chemistry, as an excellent platform for biocatalysis. It was found that pressurized CO2 is superior to conventional organic solvents for lipase-catalyzed reactions. We also achieved waste-minimization (E-factor < 0.3) in large-scale biosynthesis of chiral compounds with a continuous reactor using pressurized CO2 fluid.
Furthermore, we combined the volume-expansion capability of CO2 with a bio-based liquid intoCO2-expanded bio-based liquids as sustainable and efficient reaction media for biocatalysis (Figure 1). The lipase-catalysed transesterification in this new solvent systems resulted in increased biocatalytic activity, especially with bulky substrates such as rac-1-adamantylethanol. Currently, we are pursuing to elucidate the mechanism of the acceleration in enzymatic reactions caused by CO2. By these studies of green chemistry using enzyme and pressurized CO2, we hope to fulfill the role of scientists, to protect the environment for a sustainable future.
We have found that strains of Geotrichum candidum have many robust oxidoreductases with extremely excellent stereoselectivity. The excellent enantioselectivity can be achieved even when very challenging bulky-bulky ketones are used (Figure 2). Moreover, mutation of one key residue located within the substrate binding pocket has expanded the substrate specificity and altered the enantiopreference of this enzyme completely. Investigation of a detailed interaction between mutated residue in the binding pocket and the substrate through structural study will contribute in solving the reaction mechanism of this oxidoreductase and other oxidoreductases in general.
Let's enjoy science!! It is most important to do research to discover something new, which may be trivial, to contribute solutions to environmental problems. We are studying enzymatic reactions to construct new organic synthesis reactions.
Be part of a delightful lab team!! Teamwork is also essential to produce unique and interesting research results. Therefore, we try to make a happy, peaceful, and cooperative group. Currently, we have 12 students with great individualities from 4 different countries. Please feel free to contact me or our members for more information.
Associate Professor Tomoko Matsuda
Room 913, 914, 915, 921, J3 building, Suzukakedai campus
*Find more about the lab and the latest activities at the lab site.